Lectin Microarray Analysis

Lectin Microarray Assay Service

We offer lectin microarray assay service to help researchers to determine the glycosylation characteristics of various biological samples. The lectin microarray platform can analyze proteins, antibodies, cells, cell lysate, serum, vesicles, bacteria, or viral particles. Our experts can help you tailor a plan that best fits your research needs. From study design to product/service selection, sample collection, report, and biological interpretation, our experts guide you in every step. Your research success is our goal.

Our Process

1. Service Request
2. Study Design
3. Service Quotation
4. Shipping your samples
5. Assay
6. Data Analysis
7. Report

Knowledge Base: What is lectin microarray?

Advances in glycosylation profiling techniques have driven progression in the field of glycobiology. Lectins are a class of carbohydrate-binding proteins purified from plants or animals that can bind to different carbohydrate structural epitopes in a highly specific manner. Lectin array is a lectin-based glycan profiling technique to evaluate the entire feature of glycosylation of an intact biological sample. It is a sensitive, rapid, non-sample-destructive, and high-throughput profiling method directly applicable to glycans in native forms (e.g., on proteins, cells, and tissues). It is also in a simple assay format and requires only a small amount of a sample. Therefore, lectin array-based glycosylation profiling is one of the most widely used methods in glycobiology, especially favored by researchers who don’t have extensive experience in glycoscience.

Applications of Lectin Microarray

• Analysis of glycosylation profiles of proteins, antibodies, cells, and cell lysates.
• Carbohydrate biomarker discovery and analysis.
• Comparison of glycosylation pattern differences or alterations.
• Identification of aberrantly glycosylated cells, proteins, or antibodies.

Knowledge Base: What are the workflow steps of a lectin microarray analysis?

Step 1: Ultra-high-quality glass slides with minimal autofluorescence are coated with our patented substrates to immobilize lectins to the surface.

Step 2: Individual lectins sourced from plants are covalently attached to the microarray substrates.

Step 3: After any additional reactive sites on the coating surface are blocked, the analysis samples are introduced to the array. After incubation, any unbound samples are washed away.

Step 4: Fluorescently labeled antibodies or streptavidin bind to the samples which are bound to lectins on the array, and provide a fluorescent signal read out by a microarray scanner.

Step 5: The lectin array data are analyzed by a software tool to determine the presence of specific types of glycans or glycan motifs.