Sialoside Array

Description

Sialosides are sialic acid-containing carbohydrates that are widely present on the surface of mammalian cells. The diversity of sialosides stems from variations in sialic acid linkages, sialic acid modifications such as acetylation, and underlying glycan structures. Among these, N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) are two predominant forms of sialic acid that influence a range of biological functions.

Sialosides play essential roles in many biological processes by acting as key recognition molecules. They are involved in cell-cell communication, immune modulation, pathogen-host interactions, and cancer progression. In the immune system, sialosides interact with lectins such as Siglecs and selectins, regulating immune cell activation and inflammation. Many viruses, including influenza, and bacteria, such as Helicobacter pylori, recognize sialosides as entry receptors to establish infections. Additionally, aberrant sialylation is frequently observed in cancer, where tumor cells exploit altered sialoside expression to evade immune surveillance and enhance metastasis. Despite their biological importance, the study of sialoside interactions has been challenging due to limited access to well-defined sialylated structures.

The Sialoside Array provides a powerful tool to investigate sialoside-mediated interactions with biological samples, including proteins, antibodies, cells, cell lysates, serum, vesicles, bacteria, and viral particles. The array consists of 50 structurally defined N-glycans and LacNAc glycans, each systematically paired with Neu5Gc and Neu5Ac at the non-reducing end. This design allows researchers to compare binding preferences and functional differences between these two forms of sialic acid.

Each array contains 8 or 16 identical subarrays, enabling simultaneous analysis of multiple samples for high-throughput experimentation. The platform offers a simple assay format requiring only a small sample volume, providing reliable and reproducible glycan-binding data within a few hours.

Features

• 50 Sialoside structures;
• Unrivaled sensitivity and specificity;
• Simple assay format;
• Small sample volume;
• Customizable (select glycans for a specific microarray format)
• Assay service available;

Applications

• Evaluate binding specificities of sialoside-interacting proteins;
• Evaluate binding specificities of sialoside-interacting antibodies;
• Study virus – sialoside interactions;
• Study bacteria – sialoside interactions;
• Study vesicle – sialoside interactions;
• Study cell – sialoside interactions;