Sulfated Glycan Array

Description

Sulfated glycans are a distinct class of sugar molecules characterized by the addition of sulfate groups to their structures. These modifications play crucial roles in a variety of biological processes, including cellular communication, immune response, and the regulation of signaling pathways. Sulfated glycans are especially known for their ability to mediate interactions with proteins, such as lectins and growth factors, influencing key physiological and pathological processes. For instance, sulfated glycans have been implicated in modulating inflammation, inhibiting viral entry, and regulating tumor progression.

The biological activities of sulfated glycans are often linked to their specific structural motifs, which include the arrangement of monosaccharides, linkage types, and sulfate group positions. These motifs determine the binding affinity and specificity of sulfated glycans toward various biological targets. Due to their importance, sulfated glycans have been investigated as biomarkers for diseases and as therapeutic targets for drug development. Moreover, paired comparisons of sulfated glycans with their non-sulfated forms provide valuable insights into the functional roles of sulfation in glycan activity.

Innovative Surface Chemistry for Enhanced Multivalent Display of Sulfated Glycans

The multivalent presentation of sulfated glycans is especially important for biomolecule recognition due to the inherent negative charge associated with these glycans. This cumulative negative charge within a cluster of sulfated glycans significantly influences biomolecule recognition processes.

To achieve efficient multivalent presentation of sulfated glycans on the microarray surface, we have developed a novel approach by modifying the conventional planar NHS coating chemistry. This novel approach involves introducing proprietary highly-branched spherical polymers along with a specialized linker, which serves to bridge the polymer and NHS functional group. Unlike the traditional planar NHS coating, where one substrate unit carries only one NHS functional group, our innovative multivalent NHS coating allows for variable numbers of NHS functional groups per substrate unit. This unique spherical polymer enables the presentation of multiple glycans per substrate, facilitating effective multivalent presentation.

Features

• 46 structurally defined glycans, featuring paired sulfated and non-sulfated formats.
• Simple assay format;
• Small sample volume;
• Customizable (select sulfated glycans for a specific microarray format)
• Assay service available;

Applications

• Evaluate binding specificities of sulfated glycan-interacting proteins;
• Evaluate binding specificities of sulfated glycan-interacting antibodies;
• Study virus – sulfated glycan interactions;
• Study bacteria – sulfated glycan interactions;
• Study vesicle – sulfated glycan interactions;
• Study cell – sulfated glycan interactions;